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CAMP: Deciphering Transcriptional Regulation in a Single Cell

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Heng Xu, Baylor College of Medicine
When
12 January 2016 from 3:30 PM to 4:30 PM
Where
339 Davey Laboratory
Contact Name
Dezhe Jin
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Regulation of gene activity is mediated by transcription factors, proteins that bind specific DNA sequences to modulate mRNA production from the gene. However, a quantitative description of this process has remained elusive because of the challenges involved with measuring the underlying single-molecule events. I recently developed single-cell imaging and image-analysis methods to measure the concentration of nuclear transcription factors, number of transcription factors bound, and number of nascent mRNAs synthesized at individual gene copies. I then used theoretical analysis to decipher how transcription factor binding modulates the stochastic kinetics of mRNA production. The tools I developed provide an experimental and computational framework that can be used for deciphering transcriptional regulation in various biological systems.

 

Reference: Xu, H., et al. Combining protein and mRNA quantification to decipher transcriptional regulation. Nat. Methods 12, 739–42 (2015).

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